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3xflag tag sequence  (Addgene inc)


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    Addgene inc 3xflag tag sequence
    3xflag Tag Sequence, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/3xflag tag sequence/product/Addgene inc
    Average 93 stars, based on 5 article reviews
    3xflag tag sequence - by Bioz Stars, 2026-02
    93/100 stars

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    Map of gene constructs. (A) pTRBO-prBChE-KDEL, (B) pTRBO-prBChE, and (C) p35S-P19, 35S: Cauliflower Mosaic Virus (CaMV) promotor, RAmy3DSP : rice alpha-amylase 3D gene signal peptide, Replicase : replicase gene of tobacco mosaic virus (TMV), MP : movement protein, BChE : codon optimized sequences for the human butyrylcholinesterase gene. <t>3XFLAG</t> : specific amino acid codon sequences used for immunodetection and purification of the protein. KDEL : Codons encoding lysine, aspartic acid, glutamic acid, leucine, specifying the endoplamsmic retention sequence. P19: P19 gene from Tomato Bushy Stunt Virus (TBSV).
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    Map of gene constructs. (A) pTRBO-prBChE-KDEL, (B) pTRBO-prBChE, and (C) p35S-P19, 35S: Cauliflower Mosaic Virus (CaMV) promotor, RAmy3DSP : rice alpha-amylase 3D gene signal peptide, Replicase : replicase gene of tobacco mosaic virus (TMV), MP : movement protein, BChE : codon optimized sequences for the human butyrylcholinesterase gene. 3XFLAG : specific amino acid codon sequences used for immunodetection and purification of the protein. KDEL : Codons encoding lysine, aspartic acid, glutamic acid, leucine, specifying the endoplamsmic retention sequence. P19: P19 gene from Tomato Bushy Stunt Virus (TBSV).

    Journal: Frontiers in Plant Science

    Article Title: Transient Expression of Tetrameric Recombinant Human Butyrylcholinesterase in Nicotiana benthamiana

    doi: 10.3389/fpls.2016.00743

    Figure Lengend Snippet: Map of gene constructs. (A) pTRBO-prBChE-KDEL, (B) pTRBO-prBChE, and (C) p35S-P19, 35S: Cauliflower Mosaic Virus (CaMV) promotor, RAmy3DSP : rice alpha-amylase 3D gene signal peptide, Replicase : replicase gene of tobacco mosaic virus (TMV), MP : movement protein, BChE : codon optimized sequences for the human butyrylcholinesterase gene. 3XFLAG : specific amino acid codon sequences used for immunodetection and purification of the protein. KDEL : Codons encoding lysine, aspartic acid, glutamic acid, leucine, specifying the endoplamsmic retention sequence. P19: P19 gene from Tomato Bushy Stunt Virus (TBSV).

    Article Snippet: The 3xFLAG tag sequence (Sigma–Aldrich, St. Louis, MO, USA) was inserted between the RAmy3D signal peptide and hBChE sequences in both cassettes.

    Techniques: Construct, Immunodetection, Purification, Sequencing